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phtpp 16025  (Cayman Chemical)


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    Cayman Chemical phtpp 16025
    <t>E2</t> regulates CLDN6 expression via ERβ. a Western blot analysis of ERα and ERβ expression in MCF-7 cells treated with E2. Actin served as a loading control. b Western blot analysis of ERβ in MDA-MB-231 cells treated with E2. c MDA-MB-231 cells were transfected with either negative control (sh-NC) or three different ERβ shRNAs for 48 h and were then subjected to western blot analysis to detect the protein abundance of ERβ. Actin was used as the loading control. d ERβ knockdown abolished the CLDN6 expression induced by E2. e MDA-MB-231 cells were incubated with DPN for 24 h at the indicated concentration. CLDN6 gene and protein expression levels were detected by using semiquantitative RT-PCR and western blot. f Immunofluorescence of CLDN6 (red) was prominent along the edges of the MDA-MB-231 cells upon DPN treatment. Nuclei were stained with 4, 6-diamino-2-phenylindole (DAPI) (blue) (scale bar, 20 μm). g Tight junctions (white arrowheads) between cells were prominent in MDA-MB-231 cells after DPN treatment as observed by TEM. h The migration (scale bar, 200 μm) and invasion (scale bar, 50 μm) abilities of MDA-MB-231 cells treated with DPN were decreased. i CLDN6 knockdown rescued the migration and invasion abilities of MDA-MB-231 cells after DPN treatment. The ERβ antagonist <t>PHTPP</t> (10 μM, 24 h) ( j ) and ERβ knockdown ( k ) abolished the DPN-induced CLDN6 expression. Overexpression of ERβ induced CLDN6 upregulation in SR-BR-3 ( l ) and MDA-MB-231 ( m ) cells after treatment with DPN. Data are presented as mean ± SD. The data shown are representative results of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001
    Phtpp 16025, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phtpp+16025/phtpp+16025/pmc06694553-75-5-10
    Average 90 stars, based on 1 article reviews
    phtpp 16025 - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Estrogen receptor β inhibits breast cancer cells migration and invasion through CLDN6-mediated autophagy"

    Article Title: Estrogen receptor β inhibits breast cancer cells migration and invasion through CLDN6-mediated autophagy

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    doi: 10.1186/s13046-019-1359-9

    E2 regulates CLDN6 expression via ERβ. a Western blot analysis of ERα and ERβ expression in MCF-7 cells treated with E2. Actin served as a loading control. b Western blot analysis of ERβ in MDA-MB-231 cells treated with E2. c MDA-MB-231 cells were transfected with either negative control (sh-NC) or three different ERβ shRNAs for 48 h and were then subjected to western blot analysis to detect the protein abundance of ERβ. Actin was used as the loading control. d ERβ knockdown abolished the CLDN6 expression induced by E2. e MDA-MB-231 cells were incubated with DPN for 24 h at the indicated concentration. CLDN6 gene and protein expression levels were detected by using semiquantitative RT-PCR and western blot. f Immunofluorescence of CLDN6 (red) was prominent along the edges of the MDA-MB-231 cells upon DPN treatment. Nuclei were stained with 4, 6-diamino-2-phenylindole (DAPI) (blue) (scale bar, 20 μm). g Tight junctions (white arrowheads) between cells were prominent in MDA-MB-231 cells after DPN treatment as observed by TEM. h The migration (scale bar, 200 μm) and invasion (scale bar, 50 μm) abilities of MDA-MB-231 cells treated with DPN were decreased. i CLDN6 knockdown rescued the migration and invasion abilities of MDA-MB-231 cells after DPN treatment. The ERβ antagonist PHTPP (10 μM, 24 h) ( j ) and ERβ knockdown ( k ) abolished the DPN-induced CLDN6 expression. Overexpression of ERβ induced CLDN6 upregulation in SR-BR-3 ( l ) and MDA-MB-231 ( m ) cells after treatment with DPN. Data are presented as mean ± SD. The data shown are representative results of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001
    Figure Legend Snippet: E2 regulates CLDN6 expression via ERβ. a Western blot analysis of ERα and ERβ expression in MCF-7 cells treated with E2. Actin served as a loading control. b Western blot analysis of ERβ in MDA-MB-231 cells treated with E2. c MDA-MB-231 cells were transfected with either negative control (sh-NC) or three different ERβ shRNAs for 48 h and were then subjected to western blot analysis to detect the protein abundance of ERβ. Actin was used as the loading control. d ERβ knockdown abolished the CLDN6 expression induced by E2. e MDA-MB-231 cells were incubated with DPN for 24 h at the indicated concentration. CLDN6 gene and protein expression levels were detected by using semiquantitative RT-PCR and western blot. f Immunofluorescence of CLDN6 (red) was prominent along the edges of the MDA-MB-231 cells upon DPN treatment. Nuclei were stained with 4, 6-diamino-2-phenylindole (DAPI) (blue) (scale bar, 20 μm). g Tight junctions (white arrowheads) between cells were prominent in MDA-MB-231 cells after DPN treatment as observed by TEM. h The migration (scale bar, 200 μm) and invasion (scale bar, 50 μm) abilities of MDA-MB-231 cells treated with DPN were decreased. i CLDN6 knockdown rescued the migration and invasion abilities of MDA-MB-231 cells after DPN treatment. The ERβ antagonist PHTPP (10 μM, 24 h) ( j ) and ERβ knockdown ( k ) abolished the DPN-induced CLDN6 expression. Overexpression of ERβ induced CLDN6 upregulation in SR-BR-3 ( l ) and MDA-MB-231 ( m ) cells after treatment with DPN. Data are presented as mean ± SD. The data shown are representative results of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001

    Techniques Used: Expressing, Western Blot, Control, Transfection, Negative Control, Quantitative Proteomics, Knockdown, Incubation, Concentration Assay, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Staining, Migration, Over Expression



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    phtpp 16025  (Cayman Chemical)
    90
    Cayman Chemical phtpp 16025
    <t>E2</t> regulates CLDN6 expression via ERβ. a Western blot analysis of ERα and ERβ expression in MCF-7 cells treated with E2. Actin served as a loading control. b Western blot analysis of ERβ in MDA-MB-231 cells treated with E2. c MDA-MB-231 cells were transfected with either negative control (sh-NC) or three different ERβ shRNAs for 48 h and were then subjected to western blot analysis to detect the protein abundance of ERβ. Actin was used as the loading control. d ERβ knockdown abolished the CLDN6 expression induced by E2. e MDA-MB-231 cells were incubated with DPN for 24 h at the indicated concentration. CLDN6 gene and protein expression levels were detected by using semiquantitative RT-PCR and western blot. f Immunofluorescence of CLDN6 (red) was prominent along the edges of the MDA-MB-231 cells upon DPN treatment. Nuclei were stained with 4, 6-diamino-2-phenylindole (DAPI) (blue) (scale bar, 20 μm). g Tight junctions (white arrowheads) between cells were prominent in MDA-MB-231 cells after DPN treatment as observed by TEM. h The migration (scale bar, 200 μm) and invasion (scale bar, 50 μm) abilities of MDA-MB-231 cells treated with DPN were decreased. i CLDN6 knockdown rescued the migration and invasion abilities of MDA-MB-231 cells after DPN treatment. The ERβ antagonist <t>PHTPP</t> (10 μM, 24 h) ( j ) and ERβ knockdown ( k ) abolished the DPN-induced CLDN6 expression. Overexpression of ERβ induced CLDN6 upregulation in SR-BR-3 ( l ) and MDA-MB-231 ( m ) cells after treatment with DPN. Data are presented as mean ± SD. The data shown are representative results of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001
    Phtpp 16025, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phtpp+16025/phtpp+16025/pmc06694553-75-5-10
    Average 90 stars, based on 1 article reviews
    phtpp 16025 - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    E2 regulates CLDN6 expression via ERβ. a Western blot analysis of ERα and ERβ expression in MCF-7 cells treated with E2. Actin served as a loading control. b Western blot analysis of ERβ in MDA-MB-231 cells treated with E2. c MDA-MB-231 cells were transfected with either negative control (sh-NC) or three different ERβ shRNAs for 48 h and were then subjected to western blot analysis to detect the protein abundance of ERβ. Actin was used as the loading control. d ERβ knockdown abolished the CLDN6 expression induced by E2. e MDA-MB-231 cells were incubated with DPN for 24 h at the indicated concentration. CLDN6 gene and protein expression levels were detected by using semiquantitative RT-PCR and western blot. f Immunofluorescence of CLDN6 (red) was prominent along the edges of the MDA-MB-231 cells upon DPN treatment. Nuclei were stained with 4, 6-diamino-2-phenylindole (DAPI) (blue) (scale bar, 20 μm). g Tight junctions (white arrowheads) between cells were prominent in MDA-MB-231 cells after DPN treatment as observed by TEM. h The migration (scale bar, 200 μm) and invasion (scale bar, 50 μm) abilities of MDA-MB-231 cells treated with DPN were decreased. i CLDN6 knockdown rescued the migration and invasion abilities of MDA-MB-231 cells after DPN treatment. The ERβ antagonist PHTPP (10 μM, 24 h) ( j ) and ERβ knockdown ( k ) abolished the DPN-induced CLDN6 expression. Overexpression of ERβ induced CLDN6 upregulation in SR-BR-3 ( l ) and MDA-MB-231 ( m ) cells after treatment with DPN. Data are presented as mean ± SD. The data shown are representative results of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Estrogen receptor β inhibits breast cancer cells migration and invasion through CLDN6-mediated autophagy

    doi: 10.1186/s13046-019-1359-9

    Figure Lengend Snippet: E2 regulates CLDN6 expression via ERβ. a Western blot analysis of ERα and ERβ expression in MCF-7 cells treated with E2. Actin served as a loading control. b Western blot analysis of ERβ in MDA-MB-231 cells treated with E2. c MDA-MB-231 cells were transfected with either negative control (sh-NC) or three different ERβ shRNAs for 48 h and were then subjected to western blot analysis to detect the protein abundance of ERβ. Actin was used as the loading control. d ERβ knockdown abolished the CLDN6 expression induced by E2. e MDA-MB-231 cells were incubated with DPN for 24 h at the indicated concentration. CLDN6 gene and protein expression levels were detected by using semiquantitative RT-PCR and western blot. f Immunofluorescence of CLDN6 (red) was prominent along the edges of the MDA-MB-231 cells upon DPN treatment. Nuclei were stained with 4, 6-diamino-2-phenylindole (DAPI) (blue) (scale bar, 20 μm). g Tight junctions (white arrowheads) between cells were prominent in MDA-MB-231 cells after DPN treatment as observed by TEM. h The migration (scale bar, 200 μm) and invasion (scale bar, 50 μm) abilities of MDA-MB-231 cells treated with DPN were decreased. i CLDN6 knockdown rescued the migration and invasion abilities of MDA-MB-231 cells after DPN treatment. The ERβ antagonist PHTPP (10 μM, 24 h) ( j ) and ERβ knockdown ( k ) abolished the DPN-induced CLDN6 expression. Overexpression of ERβ induced CLDN6 upregulation in SR-BR-3 ( l ) and MDA-MB-231 ( m ) cells after treatment with DPN. Data are presented as mean ± SD. The data shown are representative results of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001

    Article Snippet: The 17β-estradiol (E2) (10006315) and PHTPP (16025) were purchased from Cayman Chemical (Denver, USA).

    Techniques: Expressing, Western Blot, Control, Transfection, Negative Control, Quantitative Proteomics, Knockdown, Incubation, Concentration Assay, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Staining, Migration, Over Expression